FastQCFastQC Report
Mon 21 Aug 2023
HHMVCDRX3_n01_S4_D28_PBMC_BCell.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHMVCDRX3_n01_S4_D28_PBMC_BCell.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83539344
Sequences flagged as poor quality0
Sequence length26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATATAAATGCGTAGGGGTTTT1750430.20953360610540586No Hit
TGTTCGTTATCGGAATTAACCAGACA1265000.15142565639490776No Hit
GCACCTGCGGCGGCTCCACCCGGGCC1190970.14256396363371013No Hit
ACCTTTAAGTTTCAGCTTTGCAACCA1123790.1345222437944928No Hit
GCTGCGGATATGGGTACGGCCCGGCG909480.1088684632237476No Hit
AGTTGGTTGATTGTAGATATTGGGCT895240.10716387717863814No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)