FastQCFastQC Report
Mon 21 Aug 2023
HHMVCDRX3_n01_S3_D7_PBMC_TCell.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHMVCDRX3_n01_S3_D7_PBMC_TCell.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108980718
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACA2833990.26004508430564754No Hit
ACCTTTAAGTTTCAGCTTTGCAACCA2816800.2584677410548901No Hit
CCTCTATATAAATGCGTAGGGGTTTT2148060.1971045923922065No Hit
TGACTTTTACTTCCTCTAGATAGTCA1879290.17244243151343525No Hit
GCACCTGCGGCGGCTCCACCCGGGCC1809270.16601744172762745No Hit
TGTTCGTTATCGGAATTAACCAGACA1631670.14972098091700956No Hit
GTCTCGTTCGTTATCGGAATTAACCA1536720.14100843050052211No Hit
GCTGCGGATATGGGTACGGCCCGGCG1519870.13946228542924446No Hit
ATTGGGCTGTTAATTGTCAGTTCAGT1275560.11704455828598964No Hit
GATCGTCTTCGAACCTCCGACTTTCG1224760.1123831832343039No Hit
CCACCAACTAAGAACGGCCATGCACC1150460.10556546342445643No Hit
AGTTGGTTGATTGTAGATATTGGGCT1117120.10250620664840912No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)