FastQCFastQC Report
Mon 21 Aug 2023
HHMVCDRX3_n01_S3_D7_PBMC_BCell.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHMVCDRX3_n01_S3_D7_PBMC_BCell.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123596032
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACA3992080.3229941880334799No Hit
ACCTTTAAGTTTCAGCTTTGCAACCA2975660.2407569200927098No Hit
CCTCTATATAAATGCGTAGGGGTTTT2669950.21602230725335908No Hit
GCTGCGGATATGGGTACGGCCCGGCG2644940.21399877950774343No Hit
GCACCTGCGGCGGCTCCACCCGGGCC2543580.20579786898013036No Hit
TGACTTTTACTTCCTCTAGATAGTCA2332860.18874877795429548No Hit
TGTTCGTTATCGGAATTAACCAGACA1911370.15464655046530945No Hit
GTCTCGTTCGTTATCGGAATTAACCA1810320.14647072164905747No Hit
GATCGTCTTCGAACCTCCGACTTTCG1557940.12605097225127745No Hit
ATTGGGCTGTTAATTGTCAGTTCAGT1554730.1257912551755707No Hit
AGTTGGTTGATTGTAGATATTGGGCT1542410.12479445942083318No Hit
GGGCAGGGACTTAATCAACGCAAGCT1356640.10976404161583439No Hit
CCACCAACTAAGAACGGCCATGCACC1341640.10855041042094296No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)