FastQCFastQC Report
Mon 21 Aug 2023
HHMVCDRX3_n01_S2_D3_PBMC_BCell.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHMVCDRX3_n01_S2_D3_PBMC_BCell.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127265777
Sequences flagged as poor quality0
Sequence length26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACA3259210.2560947708667979No Hit
GCTGCGGATATGGGTACGGCCCGGCG1699630.1335496501938616No Hit
ACCTTTAAGTTTCAGCTTTGCAACCA1673320.13148232301288665No Hit
TGACTTTTACTTCCTCTAGATAGTCA1327500.10430926768317297No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)