FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_103.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_103.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10533398
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1220471.1586669372979166TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT139100.063.31762744
TCGTATG141350.063.16216745
TATGCCG142650.062.57972348
ACGTCTG161800.061.6016615
TATCTCG144150.060.81396541
AGTCACA152100.060.0172428
CAGTCAC153800.059.7354727
GTATGCC150350.059.49287847
ACACGTC168550.059.33844813
CACGTCT168300.059.2831514
ATGCCGT151350.059.0058349
CGTATGC152450.058.90215346
TTATCTC149800.058.5912940
GTCACAT155200.058.26381729
ACATTCC154150.058.05722832
CACATTC156900.057.19493531
GTCTGAA174300.057.1448417
CACACGT177700.056.22427712
GCCGTCT159550.055.98574451
CCGTCTT160750.055.5275252
Produced by FastQC (version 0.11.2)