FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_102.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_102.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10920457
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1070950.9806824018445381TruSeq Adapter, Index 25 (97% over 44bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT148000.13552546381529637No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG111130.10176314049860732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT120350.063.03584344
TATGCCG123700.062.0996848
TCGTATG124050.061.9610745
TATCTCG126400.060.09975441
GTCACAC130850.059.875629
GTATGCC130050.059.3362847
CGTATGC130000.059.20287746
ACGTCTG146400.059.10725415
AGTCACA134850.058.611228
ATGCCGT132850.057.7984749
CAGTCAC138550.057.4441227
TCACACT136300.057.35515630
ACTGATA136350.057.2360734
TATATCT134400.056.5745139
CACGTCT153400.056.5444314
ATATCTC135350.056.20265240
ACACGTC155000.056.0494913
GTCTGAA158050.054.9267417
CTGATAT142350.054.67891335
GCCGTCT139950.054.67880251
Produced by FastQC (version 0.11.2)