FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_101.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_101.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11103746
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1746601.5729826672908405TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG197050.064.2005845
CTCGTAT195800.064.10004444
TATGCCG197350.063.97081448
AGTCACG203700.062.91647328
ACGTCTG220000.062.7991615
CACGAGT203350.062.72589531
TATCTCG199200.062.66072541
GTATGCC205050.061.74181747
GTCACGA207300.061.72567729
ACGAGTG207500.061.3042832
CAGTCAC211700.061.0283327
ACACGTC227800.060.78404213
CACGTCT228050.060.7325314
TCACGAG210250.060.6971730
ATGCCGT209150.060.414449
GGATATC207300.060.23060238
CGTATGC210350.060.1894246
ATATCTC208150.060.01779640
GATATCT210950.059.2380539
GTCTGAA234550.058.8898517
Produced by FastQC (version 0.11.2)