FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_092.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_092.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10194228
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA868640.8520900258459984TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC99900.063.3727238
CTCGTAT103400.061.55734644
TCGTATG106300.061.3041645
TACGTAA107300.060.64321536
TATGCCG107750.060.18572648
ACGTAAT107500.060.14555437
ACGTCTG128500.059.69233315
CCGTACG110700.058.87096833
CGTATGC115000.056.6366546
CAGTCAC118600.056.6056927
GTATGCC115050.056.57927347
CACGTCT136100.056.53614814
ATGCCGT117450.055.45194649
ACACGTC139150.055.34651613
TAATCTC114900.055.21772440
ACCGTAC120000.054.53980332
AGTCACC122000.054.2646628
CCGTCTT124700.052.29281652
GTCTGAA147200.052.11092417
CCAGTCA133650.052.08971826
Produced by FastQC (version 0.11.2)