FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_091.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_091.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7695891
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1617662.101978835199199TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG185350.066.0020233
TCGTATG185350.065.739345
CTCGTAT183400.065.5383644
AGTCACG190400.065.1751128
ACGTTTC188150.065.1110632
TATGCCG187700.065.00223548
TTTCGGA188500.064.8105135
GTCACGT191050.064.6829229
ACGTCTG207700.064.4793515
TTCGGAA189250.064.4633536
CACGTTT190700.064.441531
TCGGAAT191050.063.76653737
GTATGCC192250.063.55560347
CAGTCAC196650.063.5239127
CACGTCT212050.063.1728914
ATGCCGT194050.062.91272749
ACACGTC213350.062.86837413
CGTATGC196450.062.1302146
GTCTGAA216900.061.68039717
CCGTCTT200450.060.8630952
Produced by FastQC (version 0.11.2)