FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_089.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_089.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11623908
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA507340.436462504692914TruSeq Adapter, Index 20 (97% over 44bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT120150.10336454830853789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG63050.058.27041245
CTCGTAT62150.058.22813444
ACGTCTG80750.057.87246315
GTCACGT65400.056.92168429
TCACGTG65750.056.3572830
TATGCCG66200.055.33424848
AGTCACG68500.055.10021228
TATCTCG66850.053.57056441
GCCTTAT67900.053.45535337
ACACGTC89050.052.74950413
CACGTCT90200.052.00050414
CAGTCAC76500.050.9612227
CCTTATC71200.050.24983638
GTATGCC73200.050.23531747
GTCTGAA93350.050.1003917
CGTATGC74250.049.4801646
CTTATCT73250.048.8433239
ATGCCGT75650.048.37894449
TTATCTC75400.047.6340140
CACACGT103200.045.44966512
Produced by FastQC (version 0.11.2)