FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_080.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_080.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10557623
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA746760.7073183045085054TruSeq Adapter, Index 19 (97% over 40bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT119240.11294208933203997No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT115500.1093996252755No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG113930.10791254811807544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT84700.063.29451444
TCGTATG88500.062.36843545
TATGCCG89750.061.30238348
TGAAACG91600.060.5653235
GTCACGT96000.059.08011229
AGTCACG98050.058.9353628
ATGCCGT93450.058.91407849
TCACGTG95400.058.91008830
CACGTGA95750.058.40926731
GAAACGA94450.058.11734436
GTATGCC96100.057.72133647
ACGTCTG123750.057.52736315
CAGTCAC105250.056.47703627
ACACGTC126850.056.22998413
GTCTGAA126850.055.98580617
CACGTCT127600.055.73729714
GCCGTCT100450.054.64385251
CCGTCTT102850.053.4721652
CGTATGC106300.052.11902646
CTGAACT138550.051.33732619
Produced by FastQC (version 0.11.2)