FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_078.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_078.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13590322
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1256090.924253303196201TruSeq Adapter, Index 18 (97% over 40bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT147270.10836387835402282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT142700.062.54406744
TCGTATG144850.062.4713245
TATGCCG145550.062.23442548
GTCACGT148650.062.06182529
ACGTCCG146600.061.8699732
ACGTCTG197600.061.0013815
TCACGTC150300.060.89846430
AGTCACG154500.060.4698228
CGCACAT149150.060.23700737
CCGCACA153000.058.99054336
CACGTCT205000.058.8664614
ACACGTC205400.058.8355613
GTATGCC154650.058.59965547
CGTATGC155050.058.49448446
ATGCCGT157150.057.642349
GTCTGAA210000.057.51520517
CAGTCAC168400.057.21887627
GTCCGCA162350.055.9748834
GCCGTCT166350.054.359551
CTCCAGT221200.053.80334524
Produced by FastQC (version 0.11.2)