FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_077.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_077.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9351124
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG742730.7942681542881904TruSeq Adapter, Index 16 (98% over 63bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT112430.12023153580254095No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG101250.10827575380243061No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT93920.10043712392221513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT82400.062.84279642
TATGCCG85800.061.87712546
TCGTATG86650.061.23293743
ACGTCTG106800.058.90642515
CAGTCAC94550.058.48896827
GTATGCC91400.058.1988845
ATGCCGT92050.057.78637347
ACACGTC110900.057.0390413
CGTATGC93600.056.72251544
CACGTCT111400.056.50468414
AGTCACC98850.055.38569628
CGTCCAT95300.055.1703435
GTCTGAA115250.054.76849717
GCCGTCT97100.054.56784449
CCGTCTT99550.053.29619250
CATCTCG97300.052.86538739
CTGAACT122800.051.4309619
CTCCAGT121750.051.17033424
CCAGTCA112000.050.9773626
TCCAGTC123300.050.4458425
Produced by FastQC (version 0.11.2)