FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_067.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_067.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11059446
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1052670.9518288709940805TruSeq Adapter, Index 15 (97% over 40bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT157060.14201434683075445No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT138870.12556686835850547No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG135100.12215801768008994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG118750.064.0806248
CTCGTAT117100.063.5161344
TCGTATG119900.063.32673645
GTATGCC126900.060.13175247
GTCACAT129250.060.11149229
ACGTCTG147850.059.97697415
ATGCCGT127400.059.56759649
AGTCACA133250.059.08241728
CAGTCAC135300.058.6722327
CACGTCT152600.058.26801314
ACACGTC154250.057.64446613
GCCGTCT133300.056.8101651
GTCTGAA156500.056.7065417
CGTATGC134950.056.46861346
AGAATCT132800.056.1132639
ATGTCAG138700.055.76820834
CCGTCTT137150.055.44331752
CACATGT140150.055.1427431
CATGTCA145400.052.9836733
CTCCAGT165350.052.9718124
Produced by FastQC (version 0.11.2)