FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_066.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_066.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7194679
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1064251.4792181833268725TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT121700.065.1429839
TCGTATG124750.064.5400545
CTCGTAT124750.064.015144
TATGCCG126550.063.64448
TTCCGTA126050.063.39415436
TATCTCG127200.062.48465741
ACGTCTG144900.062.1737915
GTATGCC130300.061.94728547
AGTCACA133500.061.8374428
CACGTCT146850.061.41822414
CGTATGC131500.061.25338446
CAGTCAC136100.061.18926627
ATGCCGT131600.061.1514449
GTCACAG134550.060.94591529
ACACGTC149100.060.51410313
AGTTCCG133050.060.4465934
TCCGTAT132800.059.93768737
CCGTATC132300.059.76970338
GTCTGAA152200.059.2601317
CAGTTCC137300.058.75019533
Produced by FastQC (version 0.11.2)