FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_065.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_065.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8177112
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA686930.8400643161057351TruSeq Adapter, Index 13 (97% over 40bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT103810.12695191162845756No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG82560.10096474158602695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG80700.064.16485645
CTCGTAT79300.063.90868844
TATGCCG82450.062.67053248
ACGTCTG105950.061.7163115
CAGTCAC91900.060.2154827
AGTCACA90450.060.15057428
GTATGCC86450.059.97323247
CACGTCT110150.059.36271314
GTCACAG90150.059.1272129
ACACGTC111250.058.83775713
ATGCCGT88050.058.64729349
ACAGTCA90050.058.35034632
CACAGTC91300.057.5528731
GTCTGAA113850.057.4961917
GTCAACA92500.056.36038235
CAATCTC90650.055.9466640
GCCGTCT93100.055.59153751
CCGTCTT94500.054.7706452
AGCACAC120300.054.66814810
CGTATGC95100.054.62941446
Produced by FastQC (version 0.11.2)