FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_055.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_055.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6537149
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG753781.1530714689232262TruSeq Adapter, Index 12 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG89600.063.95263343
CTCGTAT88650.063.51040342
TATGCCG91050.063.08427446
ACGTCTG105100.061.1018215
TAATCTC93350.060.238438
CAGTCAC97550.060.22325527
GTATGCC95350.060.20364445
ATGCCGT95450.060.06506747
AGTCACC97100.060.003628
TGTAATC93550.059.96669436
ACACGTC108150.059.72898513
CACGTCT108000.059.6203414
CTTGTAA97050.058.5146134
GTCTGAA110450.058.32996417
CGTATGC99250.057.9774444
TTGTAAT98050.057.8135935
GTAATCT99650.056.50404437
CACACGT114450.056.3808812
TCTCGTA100350.055.9676641
CCGTCTT102200.055.590850
Produced by FastQC (version 0.11.2)