FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_054.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_054.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7301435
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1405831.9254160312322168TruSeq Adapter, Index 11 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT157250.065.6624742
TCGTATG160350.065.25317443
TATGCCG161700.064.6848446
AGTCACG169500.064.4570828
ACGTCTG201200.063.97374715
GTATGCC165700.063.3519245
CACGGCT167850.063.26496531
CAGTCAC177900.063.1782227
CACGTCT204650.062.9121314
CGTATGC168400.062.3378944
ACACGTC207050.062.3323813
ATGCCGT167800.062.24984747
GCTACAT166850.062.2178535
GTCACGG176950.061.1795829
GTCTGAA211000.061.06867217
CACACGT212900.060.602612
CCAGTCA195900.060.20755426
TCTCGTA171500.059.92452241
GGCTACA175150.059.84028634
CTACATC172550.059.5627236
Produced by FastQC (version 0.11.2)