FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_053.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_053.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11443688
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG717180.6267035592022432TruSeq Adapter, Index 10 (100% over 63bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT122630.10715951011596961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT84900.061.564142
TCGTATG86600.061.0314943
TATGCCG88500.059.87504646
ACGTCTG119000.057.35502615
AGTCACT97600.056.94317628
TATCTCG92450.056.46301739
CACGTCT122600.055.75525714
ACACGTC123000.055.60198213
GTATGCC96000.055.23372345
CAGTCAC104000.054.8987627
CTTATCT95600.054.6049637
ATGCCGT97700.054.05747247
TTATCTC98400.053.04884338
CGTATGC99900.053.0095544
GTCTGAA130900.052.2224817
GCCGTCT104850.050.3358349
CACACGT137600.049.85208512
CCAGTCA121550.049.6964426
TCCAGTC136900.049.08848625
CCGTCTT107950.048.89290250
Produced by FastQC (version 0.11.2)