FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_044.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_044.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10839955
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1381111.2740920049944857TruSeq Adapter, Index 9 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG156100.063.8478943
CTCGTAT154100.063.6487942
TATGCCG156750.063.58019346
AGTCACG164450.061.9494528
ACGTCTG190650.060.5659215
ATGCCGT165550.060.4477847
GTATGCC164700.060.427745
GTCACGA167950.060.26900529
CGTATGC166900.059.88113844
CAGTCAC175500.058.95372427
CACGTCT197000.058.75345614
ACACGTC199100.058.168413
GTCTGAA201050.057.43453617
CCGTCTT174200.057.3099850
GCCGTCT177650.056.11596349
TCAGATC174500.056.09419336
CACGATC179650.055.7521731
TCTCGTA178950.054.771141
AGATCTC178650.054.75023738
CCAGTCA196000.054.7238426
Produced by FastQC (version 0.11.2)