FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_042.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_042.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9566872
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG860950.8999284196548255TruSeq Adapter, Index 8 (100% over 63bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT107530.11239828441312898No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT103460.10814402032346623No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG103170.10784089094115612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT99300.064.3403142
TATGCCG103300.063.3818146
TCGTATG103400.063.25642843
ACGTCTG147600.060.5208415
GTCACAC112900.060.441829
CAGTCAC118900.060.1175927
GTATGCC108950.060.09522645
ATGCCGT108800.059.9862747
AGTCACA115800.059.76075428
CACGTCT151600.058.9919314
CACACTT113000.058.83466331
ACACGTC152900.058.64826213
TCACACT114500.058.63395330
GTCTGAA155150.057.5327817
GCCGTCT113900.057.14859449
CGTATGC114950.056.9596544
TTGAATC113100.056.73734336
CTTGAAT115850.056.52276235
TGAATCT112900.056.50171737
CCGTCTT116600.055.97551750
Produced by FastQC (version 0.11.2)