FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_031.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_031.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10930266
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1073470.9821078462317386TruSeq Adapter, Index 6 (100% over 63bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT109580.10025373581942104No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG109320.10001586420678142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT122400.062.7420942
TATGCCG123700.062.6359246
TCGTATG124050.062.3525343
AGTCACG126950.062.17569428
TATCTCG126800.060.37584339
GTCACGC131700.059.54165629
ACGTCTG146100.059.01520515
CAGTCAC135600.058.94780727
GTATGCC132000.058.69825445
GCCAATA132750.058.29293434
ATGCCGT133250.058.11773747
CGCCAAT133800.057.8604733
TCACGCC135700.057.5582830
CCAATAT134550.057.49008635
ATATCTC133400.057.38951538
CGTATGC135350.057.3752444
CACGCCA135900.057.29744731
CACGTCT150800.057.15314
ACGCCAA139600.055.77830532
ACACGTC156250.055.3137613
Produced by FastQC (version 0.11.2)