FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_030.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_030.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9120856
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1244981.3649815324351136TruSeq Adapter, Index 5 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT141400.063.89849542
TCGTATG144500.063.76844443
TATGCCG144900.063.4478246
GTCACAC150200.062.29187829
ACGTCTG176850.062.2515915
GTATGCC150750.061.16873645
CACAGTG152200.060.79331233
CACGTCT181650.060.7581914
ATGCCGT151550.060.6393147
ACACGTC182400.060.56508613
AGTCACA155900.060.47808528
ACACAGT153350.060.40589532
CAGTCAC159350.060.38076427
CACACAG154900.060.0022331
CGTATGC155800.059.18670344
GTCTGAA188050.058.6739617
CACACGT192450.057.45574612
GCCGTCT160000.057.2221349
CCAGTCA173300.057.15332826
CTCCAGT191050.057.1310924
Produced by FastQC (version 0.11.2)