FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_029.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_029.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10375468
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG805340.776196312301286TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT94150.063.0257242
TCGTATG97050.062.56252743
TATGCCG97650.061.99818446
ACTGACC99250.060.83255832
AGTCACT104750.060.13728
ACGTCTG123750.060.08682615
CACGTCT126500.058.9438914
CAGTCAC111050.058.24784527
GTATGCC104450.057.962245
ACACGTC130000.057.3304113
ATGCCGT106200.057.07011847
GTCTGAA132650.056.10980617
CTGACCA112400.053.7478333
ACCAATC110950.053.0814936
AGCACAC140900.053.06565510
CGTATGC115000.052.916344
GCCGTCT115200.052.5808149
CAATCTC112400.052.4875438
CACACGT145100.051.34023712
CCAGTCA131850.051.3091226
Produced by FastQC (version 0.11.2)