FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_020.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_020.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8734937
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG643350.7365250602265363TruSeq Adapter, Index 3 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG76400.062.63030643
CTCGTAT75100.062.24553742
ACTTAGG79050.061.2765132
TATGCCG78250.061.14713346
ACGTCTG98050.058.53259315
TTAGGCA84350.057.34448634
AGTCACT87000.057.06310328
GTCACTT86200.056.99202329
GTATGCC84450.056.98539445
ATGCCGT84550.056.63140547
CAGTCAC90450.056.22154227
CACGTCT103600.055.5966614
ACACGTC105300.054.7973513
TCACTTA91350.054.044330
GTCTGAA106350.053.90052417
CGTATGC89650.053.5273544
CACTTAG92350.053.1240931
CCGTCTT91100.052.29459450
GCCGTCT92500.051.7990949
CCAGTCA104450.050.86582626
Produced by FastQC (version 0.11.2)