FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_018.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_018.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10061686
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG788970.784132997193512TruSeq Adapter, Index 2 (100% over 63bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT122180.1214309410967506No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG119690.11895620674308462No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT113510.11281409497374496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT90400.062.5109242
TCGTATG92300.062.41990343
TATGCCG93900.061.31651346
ACGTCTG123000.058.51918815
ATGCCGT100100.057.7900847
CACGTCT124850.057.6520614
TATCTCG97850.057.57753839
GTATGCC100150.057.52470445
CAGTCAC108350.056.8645227
ACACGTC127100.056.82141513
AGTCACC106650.056.54149228
TGTATCT100950.055.91446337
CACCGAT105750.055.72392731
GCCGTCT103900.055.4454749
GTCTGAA130550.055.03003717
CGATGTA106150.054.79997634
CCGTCTT108200.053.37217350
CTCCAGT135750.052.39973424
CCGATGT111500.052.2935633
CGTATGC110900.052.07509244
Produced by FastQC (version 0.11.2)