FastQCFastQC Report
Mon 6 Mar 2017
HHM3YBGX2_n01_jes_017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHM3YBGX2_n01_jes_017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8520445
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG582010.6830746516173745TruSeq Adapter, Index 1 (100% over 63bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT88430.10378565908236013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT68950.061.0902642
ACGTCTG97700.059.80050315
TCGTATG73150.059.42158543
TATGCCG74250.058.5873846
CACGTCT101150.057.8290414
GTCACAT79550.056.46597329
AGTCACA81850.056.27055428
ACACGTC105550.055.4830613
CAGTCAC85300.055.3698127
GTATGCC79000.055.23906745
CGTATGC79800.055.03112844
ATGCCGT78950.054.83548747
GTCTGAA108400.053.9940917
GCCGTCT81950.052.53096849
CTGAACT111250.052.46078519
CACGATC80550.052.0405736
AGCACAC117250.050.20984310
CCGTCTT86250.049.99348450
TCTGAAC117650.049.75358218
CCAGTCA102900.049.35270726
Produced by FastQC (version 0.11.2)