FastQCFastQC Report
Fri 18 May 2018
HHLNVBCX2_l02n02.332000000dca6e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLNVBCX2_l02n02.332000000dca6e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215428843
Sequences flagged as poor quality0
Sequence length98
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA10674300.49549075468970516No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG8914740.41381366932374974No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATTG6538770.3035234237413604No Hit
GGTGCATATGGTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCT3512900.1630654443054313No Hit
GGTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATT3057710.14193596165765046No Hit
GCTAAATAAACAAGAAAGCAAAATCAACAACCGCAAAAGTCTAAGACATG2975540.13812170917150587No Hit
GGTGCAATTGCAAAGGCACAGGAGCACAGGAGCAGCTAAATAAACAAGAA2802090.13007032674821542No Hit
GCAAAATCAACAACCGCAAAAGTCTAAGACATGTATCACGAATTGAAAAT2319660.10767638946099711No Hit
GCAAAAGCCAAGGCCGGAGCAGGTGCATATGGTGCCAACCCCCCTGGCCC2300480.10678607228095266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT6931800.077.249454
AGTGGTA6939000.077.1051565
GTGGTAT7200100.074.321926
GGTATCA7391450.072.547548
AGCAGTG7451950.072.022142
TGGTATC7440650.071.9541557
AAGCAGT7681200.070.164921
GCAGTGG7683450.069.840453
GTATCAA9967700.054.0259979
TTGCGCC1913350.046.5297992
CATGGGG5176750.039.51638424-25
AGAGTAC7371050.038.44774218-19
GCAGAGT7416350.038.2435916-17
TACATGG7474400.037.4845622-23
CAACGCA7772400.036.7590912-13
ACGCAGA7792400.036.62357714-15
ATCAACG7937800.036.053910-11
CGCAGAG7514900.035.89171216-17
CAGAGTA7483550.035.88340818-19
ATGGGGG3515900.035.7652426-27
Produced by FastQC (version 0.11.2)