FastQCFastQC Report
Fri 18 May 2018
HHLNVBCX2_l02n01.331000000dca61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLNVBCX2_l02n01.331000000dca61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215428843
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT612050.017.7120361
CTGCGGA578650.017.6502631
GCGCGAT439000.017.2094291
CAATGGA570300.017.1462928
TGCTACC768700.016.9868721
GATGAAA597250.016.9096131
ATGCGAT484800.016.8920571
TGCGGGT593350.016.8504471
GACTAAC531250.016.8426631
TGGACGC605050.016.8288731
TGACGGC474100.016.8216781
CCGGGAT453900.016.7745531
TTGAACG474900.016.5868741
CTCGAAA513200.016.5557861
ACTGAAC495050.016.548371
GGGAGAT540350.016.5349481
GATCTAG712550.016.480421
GGAAAGC577200.016.4621521
CGGACGT612300.016.4335231
TGGGCGT424700.016.4012991
Produced by FastQC (version 0.11.2)