FastQCFastQC Report
Fri 18 May 2018
HHLNVBCX2_l01n02.332000000dca52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLNVBCX2_l01n02.332000000dca52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158316365
Sequences flagged as poor quality0
Sequence length98
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGCGACCCCAGGTCAGTCGGGA3044250.1922890283641871No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGTCGGG2833450.17897391719422057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT8010400.075.4045641
AGCAGTG9065250.066.532822
CAGTGGT11232700.053.6020854
GCAGTGG11282150.053.4260333
AGTGGTA11509950.052.284875
GTGGTAT13482500.044.6165926
TGGTATC13573050.044.28817
GGTATCA13764200.043.7167438
GTATCAA14036600.042.863389
CAACGCA14042950.024.06252712-13
ATCAACG14272600.023.74030310-11
AGAGTAC14131650.023.70600718-19
GCAGAGT14193750.023.6420816-17
AGTACAT14165150.023.5059620-21
ACGCAGA14410750.023.43091814-15
TACATGG14270650.023.22134822-23
TATCAAC14041450.021.47223910-11
CGCAGAG14123100.021.28696416-17
CAGAGTA14026200.021.2855718-19
TCAACGC14180850.021.23952312-13
Produced by FastQC (version 0.11.2)