FastQCFastQC Report
Fri 18 May 2018
HHLNVBCX2_l01n01.331000000dca55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLNVBCX2_l01n01.331000000dca55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158316365
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTTCATCTTTCCCTCGCGGTACT7287020.46028216981864134No Hit
TCCAGCACCGGGCAGGTGTCAGACTC3537460.2234424722927412No Hit
TTTCATCTTTCCCTCGCGGTACTTGT2569000.16227002180096795No Hit
TTTTCATCTTTCCCTCGCGGTACTTG2302220.14541895274060898No Hit
CTGGTCTGCATCGTTTATGGTTGAGA2157820.1362979752598539No Hit
CCTTCCCCTAAATGATAAGGTTCAAT1907120.12046259399652083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT473100.018.0246451
TCCACGA779850.017.9948048
AGGACGA321600.017.8473038
TGACTAG593700.017.8305871
GACTGCG642450.017.821891
GGCTCGA592100.017.812851
GGCGACT586100.017.7937221
ATAGACC494250.017.6137471
GCGTATG651650.017.6005362
CGGACTG506250.017.496711
ATGGCCA525350.017.4483683
TACGCCG551500.017.39163410
GTCGAAC519650.017.3665288
CGGAGCT555150.017.3544041
GATCTAG718550.017.282591
CGTATGT663600.017.2654843
CGAGCGA248100.017.2380756
GGGAATG382100.017.197011
ACGAGCC473200.017.1939221
ATCGAGT521100.017.1882271
Produced by FastQC (version 0.11.2)