FastQCFastQC Report
Fri 15 Mar 2019
HHLM5AFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLM5AFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25326224
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8061463.183048527091919No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN398110.15719279747348044No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGTA99000.022.52109118
GATCTGG388700.022.4271556
ATCTGGG147450.021.4940667
CGATCTG1094050.020.7512135
GGAACGT109800.020.52811217
GGGAACG116700.020.27412616
ATCTGTG78900.019.707017
TTCGGAA66200.019.0061282
CGGGAAC97900.018.5548315
CCGATCT2838700.017.4489734
TTCCGAT2910900.017.1571352
TCCGATC2934300.017.0504443
ACCGTTA66450.016.703448
CTTCCGA3037450.016.5996881
ATTCACC137800.016.3576224
CGATCTT414750.016.2754865
AGCATAC17550.016.1757742
TATTCAC140250.016.0473323
GATCTGA299300.015.973126
GATCTTG157000.015.7049596
Produced by FastQC (version 0.11.5)