FastQCFastQC Report
Fri 15 Mar 2019
HHLM5AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLM5AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25326224
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1799810.7106507468306369No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTT286350.021.2755576
ATCTGGG155100.021.2495167
GATCTGG415500.020.8693836
GAACGTA94150.020.84697318
TTCGGAA70950.020.0742532
CTCGTAT562350.019.38881539
GCTTGAA572650.019.20091256
TTGAAAA583050.019.0012858
CTTGAAA579700.018.95749557
GGAACGT107300.018.91158317
AAAGGGG573500.018.83203968
ATGCCGT589650.018.63097444
AAGGGGG581800.018.53439969
AAAAAGG592400.018.53143766
CGATCTT602550.018.5142215
TCTCGTA591600.018.47100838
AGGGGGG584500.018.4691870
GGGAACG114350.018.38854416
AAAAGGG600050.018.09165867
CGATCTG1233050.017.989555
Produced by FastQC (version 0.11.5)