FastQCFastQC Report
Fri 15 Mar 2019
HHLM5AFXY_n01_r4zt14tcs12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLM5AFXY_n01_r4zt14tcs12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTCCCGTGCTAGATCGGAAGAGCACACGTCTCCCGTGCTAGATCGGA116.666666666666664Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CAAATAACCAAACAATTACCATCCAATCACCCAAGTCAAACTGCACAAAC116.666666666666664No Hit
GCATGGGGAAGAATTCGGTTCTAAAATTCTTCTCGGCACACATCTTAGAT116.666666666666664No Hit
CCCGCGGTAGATCGGAAGAGCACACCTCTCCCGGAAGAGATCGGAAGACC116.666666666666664Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACAGAAAAAAATACACCAAAACTATCCCAACACAACGAACTACATATCTA116.666666666666664No Hit
GCACACGTCTCCCTCCAGAGATCGGAAGAGCACACGTCTCCCACCACAGA116.666666666666664Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)