FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30713690
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30119519.80654229433194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAT17050.010.0441481
CGGGAAT13400.09.2300391
GGGGAGA95150.09.1789914
ATCTACA66950.09.1436646
CGGGCAT21350.09.1352611
CATCTAC58450.09.093755
CGGGGAT23550.08.8878511
CGTGTAT15350.08.81098314-15
CCCACAT61300.08.4586491
CCCGAAT14103.6379788E-128.4344331
CCGATTT14103.6379788E-128.4244262
TCCGATC19850.08.3876921
CACGGAT17650.08.3551061
CCGATCT20150.08.2530122
AAAAGGG79700.08.05331978-79
CTCGAAT19300.07.64080951
AAAGGGG84800.07.598142680-81
CGGAAAT18200.07.57986741
CTGGAAT61050.07.5582461
CTCGGAT22050.07.33508731
Produced by FastQC (version 0.11.5)