FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n02_WT_PBS_M2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n02_WT_PBS_M2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18146425
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA246500.012.641412
ACATCTA123650.011.0973284
CGCGGAT19300.010.5899261
CCCACAT157400.010.448481
ATCTACA136550.010.1880126
AAAAGGG195800.010.09648478-79
AAGGGGG191200.09.83071880-81
TAGGGGG72600.09.77990950-51
ACCTTAC56800.09.6966826
CGGGGAT55000.09.5927271
AAAGGGG206500.09.42454780-81
GGGGGAG264100.09.4212543
AACCTTA68750.09.3921315
CATCTAC141750.09.3115015
TACAGAC134400.09.0439429
GTCGGTT26400.08.8221391
GCGTCGT215800.08.81328494-95
CCGGTTT43100.08.7122751
CCGGGAT55900.08.6730151
CGGAAAT44150.08.6127341
Produced by FastQC (version 0.11.5)