FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n02_WT_PBS_M1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n02_WT_PBS_M1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15225809
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAC51800.011.73242956
AACCTTA58100.011.1139675
GGGGGGA193550.010.8489582
ACATCTA108150.010.6248374
ATCTACA117600.09.5686086
CGCCGAT18400.09.55938151
CATCTAC123650.09.2155965
CCCACAT135450.09.0900571
CCGGAAT40000.09.0323241
AAGGGGG149800.08.97855180-81
CGCGGAT14600.08.7913751
AAAAGGG157600.08.77462378-79
CGTCGTG162300.08.70123694-95
CGGATTA31750.08.67645956-57
TTACTCG32350.08.66269760-61
CTCGCAT22700.08.5862571
TCGTGTA127700.08.5268394-95
GCGTCGT175750.08.31941694-95
CCGGGAT46600.08.2631381
CCTTACA80150.08.1751047
Produced by FastQC (version 0.11.5)