FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n02_WT_PBS_F2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n02_WT_PBS_F2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20000078
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAC66550.012.5564756
ACATCTA135200.012.3619654
AACCTTA81600.011.3460675
GGGGGGA267850.011.014062
ATCTACA156100.010.9192956
CCCACAT178050.010.679031
CATCTAC163700.010.3832975
CCGGGAT60300.09.8538631
TACAGAC148800.09.8280469
AAAAGGG211100.09.57916878-79
AAGGGGG211100.09.18603580-81
TTACTCG45550.09.17621560-61
CGGGGAT57850.09.120811
CGGGAAT44450.08.9829981
CGGATTA46000.08.7762156-57
CCGGAAT49500.08.6427331
GTCGGAT27000.08.6267281
CGCGGAT19950.08.577751
TCTACAG189450.08.5210667
CGGAAAT45200.08.5184461
Produced by FastQC (version 0.11.5)