FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n02_WT_PBS_F1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n02_WT_PBS_F1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17540116
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG183000.10433226325299104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA265750.013.2102452
ACATCTA119500.011.8807094
AAAAGGG195500.011.29072478-79
AAGGGGG192250.010.88915180-81
ATCTACA131400.010.8765936
CCCACAT154700.010.7239531
CGGGAAT40800.010.4858151
ACCTTAC55800.010.4662876
CGCGAAT10001.8189894E-1210.4578511
CATCTAC139500.010.3470115
TAGGGGG77150.010.18794450-51
AACCTTA65400.010.0914275
GGGGGAG279800.09.9620063
CGGCGAT22450.09.9517931
TACAGAC125850.09.8474539
CGCGGAT18000.09.5071381
CCGGTTT44350.09.432111
AAAAAAG362800.09.11676-77
CGGGTAT19350.09.0895121
AAAGGGG206650.08.93449680-81
Produced by FastQC (version 0.11.5)