FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n02_WT_DI244_M2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n02_WT_DI244_M2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17169986
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA229650.012.5169892
ACATCTA111350.011.0874614
CGGGGAT52800.010.4454871
TAGGGGG65000.010.04657550-51
AAAAGGG187600.09.9040478-79
AAGGGGG176600.09.90294280-81
CCCACAT146250.09.8178351
CCGGTAT19700.09.6538031
CGCGAAT10557.2759576E-119.4639291
ATCTACA123700.09.4043536
GGGGGAG251150.09.3789263
CGGCAAT27400.09.1966571
GCCGAAT27250.09.07280351
GCGTCGT212600.09.0349894-95
AAAGGGG195600.09.02603780-81
ACCTTAC51450.08.8596876
CGGGAAT39900.08.8178651
CTCGTAT18000.08.7165791
AGCGTCG244350.08.56149294-95
CGTCGTG193050.08.52148794-95
Produced by FastQC (version 0.11.5)