FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n02_WT_DI244_M1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n02_WT_DI244_M1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18451221
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA247650.011.7593422
ACATCTA117000.011.4042274
CGGGGAT58900.011.1380061
AAAAGGG195550.010.59521478-79
AAGGGGG198450.09.99817280-81
ACCTTAC54150.09.6453086
CCGGGAT60350.09.452521
CCGGAAT48100.09.3890611
TAGGGGG70450.09.30307450-51
CGGGAAT43050.09.2757661
CGCGTTT10851.2732926E-109.2009611
CGCGTAA5204.0541214E-49.1419811
GGGGGAG273000.09.1322663
CGGGCAT57650.09.07062151
CGCAAAT22550.09.0649531
CATCTAC143000.09.0644495
TACAGAC124950.09.0442129
CCCACAT159900.09.0081961
ATCTACA135350.08.9805636
AAAGGGG210600.08.92489980-81
Produced by FastQC (version 0.11.5)