FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n02_WT_DI244_F2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n02_WT_DI244_F2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17219202
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT5907.745257E-912.0847521
GGGGGGA225550.011.6262182
CCGGGAT54200.010.2608911
CGGGAAT39850.010.2581411
AAAAGGG186550.010.22793178-79
AAGGGGG178200.09.98786380-81
ACCTTAC51950.09.87041956
ACATCTA107750.09.6947294
TAGGGGG64600.09.63074550-51
AACCTTA67300.09.5237075
CGGGGAT52700.09.1098081
CGGAAAT38000.09.0063211
GGGGGAG249100.08.7500553
CGCAAAT19700.08.6862991
GCCGGAT36900.08.6307181
CGGGCAT51400.08.6003931
CGTCGTG191800.08.44184794-95
CGCGGAT16900.08.4378741
AGAGCGT301200.08.40685794-95
CCGGAAT40600.08.3124841
Produced by FastQC (version 0.11.5)