FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n02_WT_DI244_F1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n02_WT_DI244_F1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17460218
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA112700.012.3868364
GGGGGGA220850.011.5944412
ACCTTAC53500.011.5375636
ATCTACA126750.010.9385416
CGCGTAT5658.262736E-610.096861
CATCTAC136750.010.0341975
CCCACAT154100.010.0261521
AACCTTA67450.09.8550355
TACAGAC124800.09.7780399
CGCCGAT23850.09.7671
CGCGAAT11251.8189894E-129.7191621
TAGGGGG64050.09.63922450-51
AAGGGGG169900.09.46836580-81
GTCGGAT24650.09.450021
AAAAGGG179600.09.41929778-79
CCGGGAT55550.09.1570011
CGGGGAT55750.09.03887751
CGGGAAT39500.08.9061121
CGGGCAT47700.08.8700311
GGGGGAG238050.08.7376593
Produced by FastQC (version 0.11.5)