FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n02_WT_Control_M.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n02_WT_Control_M.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15177899
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAC49000.013.3715946
ACATCTA103550.012.7924254
AACCTTA59550.011.4009635
ATCTACA114850.011.2031076
CGCAAAT16300.010.7886761
CCGGAAT36150.010.78101351
CATCTAC120050.010.7175235
TACAGAC111550.010.6838379
CCCACAT139300.010.645291
GGGGGGA195550.09.5952022
CGCCGAT19950.09.2912851
CGGGGAT45200.09.2533361
AAAAGGG156400.09.17677178-79
CGGCAAT22800.09.1721651
AAGGGGG149900.09.131453580-81
CGGGAAT33600.08.91159251
GGCGATT24250.08.819721
TAGGGGG58150.08.6979650-51
TCGGATC36150.08.6726252
GAAACCT103550.08.5749533
Produced by FastQC (version 0.11.5)