FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n02_WT_Control_F.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n02_WT_Control_F.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15920565
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA110250.013.953234
ACCTTAC51050.013.2995336
ATCTACA116250.012.0074416
CATCTAC126850.011.0787435
CGGCAAT24300.010.9539121
AACCTTA63600.010.8243075
CCCACAT141700.010.5328831
TACAGAC116400.010.483079
GGGGGGA210200.010.0564122
GCCGGTT32450.09.6675561
TCTACAG147650.09.6469967
CCGGGAT50500.09.4123071
CGCATAT9408.74752E-99.1019011
AAAAGGG160000.08.9402778-79
CACATCT179500.08.8089393
AAGGGGG155400.08.79274580-81
GTCGGAT24450.08.7482491
GAAACCT115250.08.6933143
TCGTGTA127050.08.68317194-95
CCGGTTT39050.08.642211
Produced by FastQC (version 0.11.5)