FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n02_KO_PBS_M2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n02_KO_PBS_M2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17745259
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA262900.015.9733322
ACATCTA125800.012.7949154
GGGGGAG265450.012.5576823
TAGGGGG73150.012.36820750-51
ACCTTAC56600.012.0795916
ATCTACA137150.011.94343956
CATCTAC143200.011.6707415
TATAGGG44300.011.57800948-49
AAAGGGG211800.011.54494180-81
AACCTTA67350.011.2792755
CCCACAT155150.011.1209911
TACAGAC138300.010.7457249
AAAAGGG200550.010.59207478-79
AAGGGGG199350.010.37063480-81
GGGGAGA220100.010.3331774
CGGGGAT56650.010.2364311
AGGGGGG271250.010.20624182-83
CGCGTAA6152.0766092E-610.0474741
AAAAAGG268350.09.97017978-79
TCTACAG163900.09.9072267
Produced by FastQC (version 0.11.5)