FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n02_KO_PBS_M1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n02_KO_PBS_M1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19629822
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA280550.014.5107942
ACATCTA133750.012.6728974
GGGGGAG287100.011.7261813
TAGGGGG78800.011.72231350-51
ACCTTAC57750.011.016636
CCCACAT167000.010.98667051
ATCTACA152350.010.9385716
CGGGGAT62100.010.4098041
AAAGGGG226700.010.37767980-81
TATAGGG47550.010.33721748-49
AAAAGGG210850.010.311686578-79
CATCTAC160000.010.1778155
TACAGAC150850.010.1663569
GGGGAGA236500.010.0177654
AACCTTA74000.09.9444435
AAGGGGG213350.09.90206180-81
AGGGGGG282350.09.578135582-83
CCGGAAT51700.09.5617651
CCGGGAT56550.09.4981951
GGGGGGT236050.09.40357314-15
Produced by FastQC (version 0.11.5)