FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n02_KO_PBS_F2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n02_KO_PBS_F2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16384772
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA113300.013.4518834
GGGGGGA213800.012.1307922
ATCTACA127550.011.6140076
CCCACAT144000.011.2561681
CATCTAC134200.011.073715
ACCTTAC47700.010.6505836
TACAGAC126950.010.5097459
TAGGGGG62650.010.42330850-51
AAAAGGG177700.09.93521478-79
AAGGGGG171100.09.88855880-81
CGGCGAT19250.09.6301391
CGCGATT5506.792997E-59.5066761
CGGGGAT49950.09.2307061
CGCGGAT17800.09.0794111
CGGATCG12051.0913936E-119.0630683
CGGGCAT46750.09.0491351
CGGGAAT39200.08.9731391
TCTACAG159900.08.848767
TCGTGTA172150.08.78322894-95
GGGGGGT190850.08.78231614-15
Produced by FastQC (version 0.11.5)