FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n02_KO_PBS_F1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n02_KO_PBS_F1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15182697
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA107900.014.4336284
ATCTACA116950.013.1939926
ACCTTAC48550.012.8107676
GGGGGGA210950.012.4080662
TACAGAC112400.012.1656019
CATCTAC125600.011.9450415
CCCACAT132700.011.74912551
AACCTTA58500.011.6868325
TAGGGGG59450.011.46339650-51
AAGGGGG157500.011.08985180-81
AAAAGGG163650.011.07864578-79
CGCGTAT4851.702274E-510.7808641
CCGGGAT46900.010.6418981
CGGGAAT31850.010.5962341
CGGCAAT22550.010.3288551
GGGGGAG220100.09.7082913
TCTACAG143650.09.6509877
AAAGGGG172200.09.49454680-81
AGGGGGG218800.09.31886982-83
TATAGGG35500.09.16363148-49
Produced by FastQC (version 0.11.5)