FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n02_KO_DI244_F2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n02_KO_DI244_F2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18233659
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA318500.019.5547332
GGGGGAG300900.016.365773
GGGGAGA242400.015.1025134
TAGGGGG79650.013.83325450-51
AAAGGGG250950.013.66447980-81
TATAGGG45900.012.3125748-49
ACATCTA120000.012.0682984
AAAAAGG315500.012.00479278-79
GGGGGGT261300.011.6840414-15
AGGGGGG336750.011.59460282-83
GGGAAAA255550.011.17618922-23
AAAAGGG247700.011.11790978-79
GGGGGTT125800.011.07768414-15
AAGGGGG238950.011.0489580-81
CGGGAAT40600.011.0068251
ACCTTAC58800.010.9816816
AGAGCGG86350.010.8320918
GTCGGAT23050.010.7248861
GAGCGGG108150.010.6242539
GGGAGAG296050.010.3280535
Produced by FastQC (version 0.11.5)